diff --git a/CHANGELOG.md b/CHANGELOG.md
index f30f41743..9cfc73cab 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -18,6 +18,7 @@
  * `ERC721`: removed redundant require statement. ([#3434](https://github.com/OpenZeppelin/openzeppelin-contracts/pull/3434))
  * `PaymentSplitter`: add `releasable` getters. ([#3350](https://github.com/OpenZeppelin/openzeppelin-contracts/pull/3350))
  * `Initializable`: refactored implementation of modifiers for easier understanding. ([#3450](https://github.com/OpenZeppelin/openzeppelin-contracts/pull/3450))
+ * `Proxies`: remove runtime check of ERC1967 storage slots. ([#3455](https://github.com/OpenZeppelin/openzeppelin-contracts/pull/3455))
 
 ### Breaking changes
 
diff --git a/contracts/proxy/ERC1967/ERC1967Proxy.sol b/contracts/proxy/ERC1967/ERC1967Proxy.sol
index 95a1587f6..c20691c7c 100644
--- a/contracts/proxy/ERC1967/ERC1967Proxy.sol
+++ b/contracts/proxy/ERC1967/ERC1967Proxy.sol
@@ -20,7 +20,6 @@ contract ERC1967Proxy is Proxy, ERC1967Upgrade {
      * function call, and allows initializing the storage of the proxy like a Solidity constructor.
      */
     constructor(address _logic, bytes memory _data) payable {
-        assert(_IMPLEMENTATION_SLOT == bytes32(uint256(keccak256("eip1967.proxy.implementation")) - 1));
         _upgradeToAndCall(_logic, _data, false);
     }
 
diff --git a/contracts/proxy/beacon/BeaconProxy.sol b/contracts/proxy/beacon/BeaconProxy.sol
index e2d192c21..4a9c001da 100644
--- a/contracts/proxy/beacon/BeaconProxy.sol
+++ b/contracts/proxy/beacon/BeaconProxy.sol
@@ -28,7 +28,6 @@ contract BeaconProxy is Proxy, ERC1967Upgrade {
      * - `beacon` must be a contract with the interface {IBeacon}.
      */
     constructor(address beacon, bytes memory data) payable {
-        assert(_BEACON_SLOT == bytes32(uint256(keccak256("eip1967.proxy.beacon")) - 1));
         _upgradeBeaconToAndCall(beacon, data, false);
     }
 
diff --git a/contracts/proxy/transparent/TransparentUpgradeableProxy.sol b/contracts/proxy/transparent/TransparentUpgradeableProxy.sol
index 10808d58d..aa373fa37 100644
--- a/contracts/proxy/transparent/TransparentUpgradeableProxy.sol
+++ b/contracts/proxy/transparent/TransparentUpgradeableProxy.sol
@@ -36,7 +36,6 @@ contract TransparentUpgradeableProxy is ERC1967Proxy {
         address admin_,
         bytes memory _data
     ) payable ERC1967Proxy(_logic, _data) {
-        assert(_ADMIN_SLOT == bytes32(uint256(keccak256("eip1967.proxy.admin")) - 1));
         _changeAdmin(admin_);
     }